Leukoagglutination reported as platelet clumps

Leukoagglutination reported as platelet clumps
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Author: Ariane Vienny Grob and Anne Angelillo-Scherrer
Category: Laboratory Hematology > Basics of automated cell counts  
Published Date: 07/28/2012

A 13-year-old girl with juvenile rheumatoid arthritis presented for a routine examination. Except for a short episode of a cough 1 week prior, she had no complaints. The chest examination was normal. Blood cell count was performed on an automated analyzer using an ethylenediaminetetraacetic acid (EDTA) blood specimen and showed normal hemoglobin and leukocytes (7.2 × 109/L), but a mild thrombocytosis (467 × 109/L). Platelet clumps were flagged by the cell counter. However, when the peripheral smear was reviewed, there was leukocyte agglutination (shown, left) rather than clumps of platelets. The cold agglutinin test was positive with relative anti-I specificity. Serology for Mycoplasma pneumoniae was positive (complement fixation test 1:32, reference value = 1:16; microparticles agglutination 1:1280, reference value = 1:40). When the blood was warmed at 37°C, the smear no longer showed leukoagglutination (shown, right). The cause of leukoagglutination in this case was uncertain, but co