Dysplastic changes of peripheral eosinophils in acute myeloid leukemia with myelodysplastic syndrome–related features

Dysplastic changes of peripheral eosinophils in acute myeloid leukemia with myelodysplastic syndrome–related features
#00060677
Author: Sunyoung Lee; John O. Mascarenhas
Category: Myeloid Neoplasms and acute leukemia (WHO 2016) > Acute Myeloid Leukemia > Acute myeloid leukemia with myelodysplasia-related changes
Published Date: 05/03/2016

A 41-year-old man presented with epistaxis for 1 day and was found to have leukocytosis (23 200/µL) with 38% blasts, anemia (9.3 g/dL), and thrombocytopenia (7000/µL). Bone marrow aspiration and biopsy showed sheets of blasts comprising 90% of the overall cellularity. Flow cytometry and immunohistochemical stains demonstrated a population of cells that expressed CD13 +, CD33 +, CD34 partial +, CD45 partial +, CD117 +, myeloperoxidase +, and HLA-DR +. Monosomy 7 and FMS-like tyrosine kinase 3–internal tandem duplication were detected. Acute myeloid leukemia with myelodysplastic syndrome (MDS)–related features was diagnosed based on an MDS-related cytogenetic abnormality (monosomy 7).Peripheral smears revealed dysplastic features of eosinophils. Cytoplasmic granules of eosinophils imaged on transmission electron microscopy (TEM) revealed a high degree of heterogeneity in size with totally shattered electron-rich cores (panels A-B). This finding on TEM is a different morphologic feature from that shown in piecemeal degranulation recognized in eosinophils during inflammatory responses. Karyorrhexis is a fragmentation of the nucleus, listed as a dysplastic feature in the erythroid lineage (dyserythropoiesis) in the World Health Organization classification, but the smear results from this patient revealed a rare finding of karyorrhexis of eosinophils (dysgranulopoiesis, panel C).