Atypical FISH signal pattern in APL (Acute Promyelocytic Leukemia)

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Atypical FISH signal pattern in APL (Acute Promyelocytic Leukemia)

#00061451

Author: Mr Anil Kumar Yadav, Dr Manorama Bhargava
Category: Myeloid Neoplasms and acute leukemia (WHO 2016) > Acute Myeloid Leukemia > Acute Myeloid Leukemia with recurrent genetic abnormalities > AML with t(15;17)(q22;q12); PML-RARA (and variants)
Published Date: 05/29/2018

APL patient cells showing positivity for PML/RARA fusion by FISH. FISH with the LSI PML/RARA Dual Color Dual Fusion Translocation Probe (Zytovision) on a peripheral blood cell sample, showing two interphase nuclei with one nuclei showing one fusion signal(PML/RARA), two red(PML) and two green(RARA) signals and other showing two green and two orange(2G2R)-negative for t(15;17).The atypical FISH signal pattern (1F2R2G) suggests involvement of third chromosome i.e. "V" other than 15 and 17. It is called as three way chromosomal translocation. It can be confirmed by Karyotype (Conventional Chromosomal Analysis-CCA) analysis. This type of aberrant result suggest the importance of combinig both Karyotype & FISH approach for disease categorization.

Reference- Image no #00061450 for standard pattern by FISH in APL.

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