Figure 1: Cytology of BIA-ALCL
As in this case, BIA ALCL often is first identified in cytological specimens. The cytology preparation (left) and cell block (right) both show large pleomorphic cells in a background of reactive inflammatory cells. Although BIA ALCL is rare, the development of peri-implant seromas is not uncommon. Therefore, these specimens should be examined carefully for atypical cells, the presence of which may be much more subtle than in the presented case.
Figure 2: Phenotype of BIA-ALCL
Immunohistochemistry on sections of the cell block shows the tumor cells to be negative for CD20 and CD45. They show weak and focal expression of CD3. CD3 and CD45 highlight reactive cells in the background. The tumor cells show strong, uniform staining for CD30 with a membranous and Golgi pattern of staining, as typically seen in ALCL. Immunohistochemistry for ALK, a surrogate marker for the presence of an ALK rearrangement, is negative.
Figure 3: T-cell markers in BIA-ALCL
The tumor cells express CD4 and CD43. There is loss of multiple other T-cell antigens. CD2 is only focally expressed, and CD5 and CD7 are negative. CD8 is negative. Many ALCLs lack expression of T-cell receptor-associated proteins. Here, neither an ab nor a gd phenotype can be demonstrated, as shown by negativity for bF1 and TCRd, respectively. Although not performed in this case, BIA ALCLs typically express cytotoxic markers such as TIA1, granzyme B, and/or perforin.
Figure 4: Genetics of BIA-ALCL
The image shows fluorescence in situ hybridization (FISH) using a breakapart probe to the DUSP22 locus on 6p25.3. Two tumor cell nuclei each show a normal signal pattern of two red-green fusion signals, indicating the absence of a DUSP22 rearrangement. DUSP22 rearrangements are seen in up to 30% of systemic or primary cutaneous ALCLs, but have been absent in reported cases of BIA ALCL, as have rearrangements of ALK and TP63.
Figure 5: BIA ALCL in a capsulectomy specimen
Although BIA ALCL may be diagnosed in cytology specimens as above, it also may also be identified first in capsulectomy specimens at the time of implant removal. These images show such a case. The low-power image shows the fibrous capsule surrounding the breast implant. The inner surface of the capsule is covered by fibrinous material and a layer of atypical cells. The high-power image demonstrates these large pleomorphic cells.