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Figure 3: Polyacrylamide gel electrophoresis of PCR-RFLP for Chuvash polycythemia. The wild type VHL allele produces a 268 bp PCR product, which is digested by the restriction enzyme Fnu4HI into 187 bp and 81 bp bands (lanes 1, 2, 3, 5, 6, 7). However, the VHL:c.598C>T substitution in Chuvash polycythemia patients results in an uncut 268 bp band (lane 4). Heterozygotes will show all the 3 bands (268bp, 187bp, and 81bp).