Colonoscopy in Indolent T-cell Lymphoproliferative Disorder
Colonoscopy depicts polyps extending from ileum to rectum.
Figure 1: Colonic Biopsy H&E in Indolent T-cell Lymphoproliferative Disorder
These slides are from the second colonic biopsy. All three colonic biopsies revealed an almost similar morphology of a lymphoid infiltrate composed of medium sized lymphocytes expanding the lamina propria and displacing the crypts. There was no evidence of crypt invasion, cryptitis, or significant distortion of crypt architecture. The lymphoid cells had mature nuclear chromatin, inconspicuous nucleoli, and a moderate amount of eosinophilic to clear cytoplasm. Mitotic figures were hard to find, and an infiltrate of eosinophils also accompanied the lymphoid cells.
Figure 2: Colonic Biopsy CD20, CD3, CD5, CD2, & CD7 in Indolent T-cell Lymphoproliferative Disorder
More immunostains were preformed on the second biopsy than were preformed on the first one. The image displays positive CD3, CD2, and CD7 as well as negative CD20 and CD5. The immunoprofile was remarkable for loss of CD5.
Figure 3: Colonic Biopsy CD4 & CD8 in Indolent T-cell Lymphoproliferative Disorder
More immunostains were performed on this second biopsy than on the first biopsy. The images below depict negative CD4 and positive CD8 immunostains. The immunoprofile was remarkable for CD8 restricted population of T-cells.
Figure 4: T-cell Receptor Gene Rearrangement Studies in Indolent T-cell Lymphoproliferative Disorder
Clonality analysis with BIOMED primer sets was carried out on thesecond and third biopsies at the time of diagnosis. Both results showed IgH in germline and rearrangements in beta and gamma chains of T-cell receptors. The T-cell receptor gene rearrangement studies showed TCR gamma & TCR beta chains rearranged.
Figure 5: Colonic Biopsy H&E Low Power in Indolent T-cell Lymphoproliferative Disorder
The third biopsy depicted medium sized lymphoid infiltrate in the lamina propria. The infiltrate displaces the glands, but does not invade the epithelium. There is no overt destruction. The lymphoid cells have mature nuclear chromatin, inconspicuous nucleoli, scant cytoplasm with an infiltrate of eosinophils.
Figure 6: Colonic Biopsy H&E High Power in Indolent T-cell Lymphoproliferative Disorder
The third biopsy depicted medium sized lymphoid infiltrate in the lamina propria. The infiltrate displaces the glands, but does not invade the epithelium. There is no overt destruction. The lymphoid cells have mature nuclear chromatin, inconspicuous nucleoli, scant cytoplasm with an infiltrate of eosinophils.
Figure 7: Colonic Biopsy CD20, CD3, CD5, & CD2 in Indolent T-cell Lymphoproliferative Disorder
The third biopsy immunostians depicted below were negative for CD20 and CD5 while they were positive for CD3 and CD2.
Figure 8: Colonic Biopsy CD4 & CD8 in Indolent T-cell Lymphoproliferative Disorder
The third biopsy immunostains depicted below were negative for CD4 and positive for CD8.
Figure 9: Colonic Biopsy Immunostains in Indolent T-cell Lymphoproliferative Disorder
The third biopsy immunostains depicted below were negative for CD56 and Granzyme B. They were positive for TIA-1 and TCR-BF1.
Figure 10: Colonic Biopsy CD8 & MBI-1 in I
CD8 immunostain was positive, and MIB 1 labeling amounted to < 5%.
